Neuro-CASA Refuses to Guess When the Microscope Is Uncalibrated

Field notes

The page frames CASA velocity as s × pixel velocity, where s is the microscope’s pixel-to-micrometre scale factor. Its example reports s = 0.184 µm/px and a VCL interval of 81.1–157.6 µm/s when calibration is verified; an unknown scale produces RET instead of a clinical grade and exposes a QC-FAIL research result. Validation material covers three datasets, four cohorts, and phase-contrast, confocal, and bright-field imaging.